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From: Kuch
Date: 5/9/2002
Time: 1:39:58 PM
Remote Name: 66.57.114.27
We all want that latest and greatest breakthroughs and I think events of years past get overlooked. The Bioamide abstract states they tried to grow hairs from cultured dermal papilla cells...I thus assume ONLY that type of cell. It is not surprising that they had poor results if you read Jahoda's 1994 publication on the ingredients for producing a hair follicle...DP cells are only one ingredient of his successful culture. What's even MORE interesting is that Jahoda reports using an EMPTY RAT WHISKER FOLLICLE (Only the intact membrane) to place his cocktail of cells into...I.E. an in-vivo "container" (to quote the abstract) akin to the artificial well that Bioamide has tried! Here is the article for those who haven't seen it:
J Dermatol Sci 1994 Jul;7 Suppl:S84-97
Hair follicle reconstruction in vitro.
Reynolds AJ, Jahoda CA.
Department of Biological Sciences, University of Durham, UK.
The in vitro creation of a follicular structure capable of hair growth from cultured adult cells has long been an aim of researchers in hair biology. Basal outer root sheath cells (ORS) attached to the hair follicle glassy membrane (GM) were isolated and cultured, where they revealed greater replicative potential and longevity than ORS cells from plucked fibres. Hair follicles were reconstructed in vitro using the collagenous shells of rat vibrissa follicles as natural containers for 4 hair follicle cell types. Basal ORS cells were initially seeded onto the residual vibrissa GM, and cultured dermal papilla, dermal sheath and germinative epidermal cells were then added. Histology revealed that after 2 or 3 weeks in combined culture, cell interactions and tissue morphogenesis had resulted in the formation of irregular but recognizable hair fibres, produced from unusual bulb structures. To our knowledge this represents the first example of adult cell populations instigating the de novo creation of hair fibres in a culture dish. While the usefulness of the current methodology relates immediately to hair growth research, the generation of hair follicles and fibres in vitro establishes the enormous potential of this type of interactive work for practical purposes.
Kuch
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